机构：[1]Department of Endocrinology, the Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang 050011, China临床科室内分泌科河北医科大学第四医院[2]Department of Vascular Surgery, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China
The therapeutic effect of transplantation of human umbilical cord blood cell-derived mononuclear cells (HUCMNCs) on treating in-stent restenosis (ISR) after endovascular interventional therapy (EIT) was evaluated in preclinical rabbit model of type 2 diabetes mellitus (T2DM)-related peripheral artery disease (PAD). HUCMNCs were transplanted to T2DM rabbits subjected to femoral artery occlusion surgery and received EIT. Serum concentration of soluble vascular endothelial cadherin (VE-cad) and plasma concentration of lipoprotein-associated phospholipase A2 (Lp-PLA2) were determined with enzyme-linked immunosorbent assay before and after the transplantation. The injury and the recovery of right femoral artery at stenting site were evaluated with Hematoxylin and Eosin (HE) staining. HUCMNCs purified from umbilical cord blood were 100% CD45(+) and 96.5% CD34(-) with round or oval morphology and adherent growth pattern. The soluble VE-cad and Lp-PLA2 were significantly attenuated after HUCMNC transplantation. The intimal area and the ratio between intimal area and medium film area in the dilated occlusion site were also dramatically decreased 4 weeks after receiving HUCMNCs. HUCMNC transplantation is effective in protecting vascular endothelial function and preventing ISR after EIT in T2DM rabbits suffering from PAD.
基金：
Department of Health of Hebei Province (No.
H20150760).
第一作者机构：[1]Department of Endocrinology, the Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang 050011, China
通讯作者：
推荐引用方式(GB/T 7714)：
Ding Haixia,Zhang Tong,Du Yaping,et al.HUCMNCs protect vascular endothelium and prevent ISR after endovascular interventional therapy for vascular diseases in T2DM rabbits[J].MOLECULAR AND CELLULAR BIOCHEMISTRY.2017,433(1-2):161-167.doi:10.1007/s11010-017-3024-y.
APA：
Ding, Haixia,Zhang, Tong,Du, Yaping,Liu, Bei,Liu, Yueqin&Wang, Fujun.(2017).HUCMNCs protect vascular endothelium and prevent ISR after endovascular interventional therapy for vascular diseases in T2DM rabbits.MOLECULAR AND CELLULAR BIOCHEMISTRY,433,(1-2)
MLA：
Ding, Haixia,et al."HUCMNCs protect vascular endothelium and prevent ISR after endovascular interventional therapy for vascular diseases in T2DM rabbits".MOLECULAR AND CELLULAR BIOCHEMISTRY 433..1-2(2017):161-167
