机构:[1]Hebei Med Univ, 361 East Zhongshan Rd, Shijiazhuang 050017, Hebei, Peoples R China[2]Hebei Med Univ, Hosp 4, Shijiazhuang 050011, Hebei, Peoples R China河北医科大学第四医院[3]Hebei Univ Chinese Med, Dept Pharmacol, Shijiazhuang 050200, Hebei, Peoples R China[4]Hebei Univ Chinese Med, Dept Med Chem, Shijiazhuang 050200, Hebei, Peoples R China[5]Hebei Gen Hosp, Dept Infect Dis, 348 Heping West Rd, Shijiazhuang 050051, Hebei, Peoples R China
Tannic acid (TA) is a group of water-soluble polyphenolic compounds that occur mainly in plant-derived feeds, food grains and fruits. Many studies have explored its biomedical properties, such as anticancer, antibacterial, antimutagenic, antioxidant, antidiabetic, antiinflammatory and antihypertensive activities. However, the effects of TA on the L-type Ca2+ current (ICa-L) of cardiomyocytes remain undefined. The present study examined the effects of TA on ICa-L using the whole-cell patch-clamp technique and on intracellular Ca2+ handling and cell contractility in rat ventricular myocytes with the aid of a video-based edge detection system. Exposure to TA resulted in a concentration- and voltage-dependent blockade of ICa-L, with the half maximal inhibitory concentration of 1.69M and the maximal inhibitory effect of 46.15%. Moreover, TA significantly inhibited the amplitude of myocyte shortening and peak value of Ca2+ transient and increased the time to 10% of the peak. These findings provide new experimental evidence for the cellular mechanism of action of TA and may help to expand clinical treatments for cardiovascular disease. Copyright (c) 2016 John Wiley & Sons, Ltd.
基金:
Hebei Science and Technology Bureau Fund [C2011206025]; Nature Fund of Hebei Province, China [QN20131046]
第一作者机构:[1]Hebei Med Univ, 361 East Zhongshan Rd, Shijiazhuang 050017, Hebei, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Hebei Med Univ, 361 East Zhongshan Rd, Shijiazhuang 050017, Hebei, Peoples R China[3]Hebei Univ Chinese Med, Dept Pharmacol, Shijiazhuang 050200, Hebei, Peoples R China[*1]Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China
推荐引用方式(GB/T 7714):
Zhu Fengli,Chu Xi,Wang Hua,et al.New Findings on the Effects of Tannic Acid: Inhibition of L-Type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes[J].PHYTOTHERAPY RESEARCH.2016,30(3):510-516.doi:10.1002/ptr.5558.
APA:
Zhu, Fengli,Chu, Xi,Wang, Hua,Zhang, Xuan,Zhang, Yuanyuan...&Zhang, Jianping.(2016).New Findings on the Effects of Tannic Acid: Inhibition of L-Type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes.PHYTOTHERAPY RESEARCH,30,(3)
MLA:
Zhu, Fengli,et al."New Findings on the Effects of Tannic Acid: Inhibition of L-Type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes".PHYTOTHERAPY RESEARCH 30..3(2016):510-516