A combinatory use of adenoviruses expressing melanoma differentiation-associated gene-7 and replication-competent adenoviruses produces synergistic effects on pancreatic carcinoma cells
机构：[1]Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室血液内科河北医科大学第四医院[2]Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuo-ku, Chiba 260-8717, Japan[3]Department of Respirology, Graduate School of Medicine, Chiba University, Chiba, Japan[4]Department ofMolecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan Tumor Biol. (2015) 36:8137–8145 DOI 10.1007/s13277-015-3555-3[5]Department of Immunology, Hebei Medical University, Shijiazhuang, China[6]Cell Therapy Center, The First Hospital of Hebei Medical University, Shijiazhuang, China[7]Department of Thoracic Diseases, Chiba Cancer Center, Chiba, Japan[8]Department of Surgery, School of Medicine, Toho University, Tokyo, Japan[9]Department of Pathology, Tokyo Women’s Medical University Yachiyo Medical Center, Yachiyo, Japan
Type 5 adenoviruses expressing mda-7 gene (Ad-mda-7) induced cell death in various kinds of human tumors, but pancreatic carcinoma cells were relatively resistant to Ad-mda-7-mediated cytotoxicity. We then examined whether infection of Ad-mda-7 together with replication-competent Ad produced combinatory cytotoxic effects. We prepared replication-competent Ad, defective of the E1B55kDa gene or activated by a transcriptional regulatory region of the midkine or the survivin gene of which the expression was up-regulated in human tumors. Type 5 Ad bearing the exogenous regulatory region were further modified by replacing the fiber-knob region with that of type 35 Ad. Pancreatic carcinoma cells were infected with replication-incompetent Ad-mda-7 and the replication-competent Ad. Combinatory effects were examined with the CalcuSyn software and cell cycle analyses. Ad-mda-7 and the replication-competent Ad achieved cytotoxicity to pancreatic carcinoma. A combinatory use of Ad-mda-7 and either Ad defective of the E1B55kDa gene or Ad activated by the regulatory region produced synergistic cytotoxic effects. Cell cycle analyses demonstrated that the combination increased sub-G1 populations. These data collectively suggest that expression of MDA-7 augments cytotoxicity of replication-competent Ad and achieves adjuvant effects on Ad-mediated cell death.
基金：
Ministry of Education, Culture, Sports, Science and Technology of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Ministry of Health, Labor and Welfare of JapanMinistry of Health, Labour and Welfare, Japan; Nichias Corporation
第一作者机构：[1]Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China
共同第一作者：
通讯作者：
通讯机构：[2]Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuo-ku, Chiba 260-8717, Japan[4]Department ofMolecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan Tumor Biol. (2015) 36:8137–8145 DOI 10.1007/s13277-015-3555-3
推荐引用方式(GB/T 7714)：
Ma Guangyu,Zhong Boya,Okamoto Shinya,et al.A combinatory use of adenoviruses expressing melanoma differentiation-associated gene-7 and replication-competent adenoviruses produces synergistic effects on pancreatic carcinoma cells[J].TUMOR BIOLOGY.2015,36(10):8137-8145.doi:10.1007/s13277-015-3555-3.
APA：
Ma, Guangyu,Zhong, Boya,Okamoto, Shinya,Jiang, Yuanyuan,Kawamura, Kiyoko...&Tagawa, Masatoshi.(2015).A combinatory use of adenoviruses expressing melanoma differentiation-associated gene-7 and replication-competent adenoviruses produces synergistic effects on pancreatic carcinoma cells.TUMOR BIOLOGY,36,(10)
MLA：
Ma, Guangyu,et al."A combinatory use of adenoviruses expressing melanoma differentiation-associated gene-7 and replication-competent adenoviruses produces synergistic effects on pancreatic carcinoma cells".TUMOR BIOLOGY 36..10(2015):8137-8145
