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Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells

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机构: [1]College of Life Science, Hebei University, Baoding 071002, China [2]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China [3]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China. [4]Research Centre, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China [5]Clinical Laboratory, Bethune International Peace Hospital, Shijiazhuang 050082, China [6]Hebei Provincial Tumor Institute, Shijiazhuang 050011, China [7]College of Life Science, Agricultural University of Hebei, Baoding 071001, China
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关键词: HeLa cells spider venom cell proliferation cytotoxicity cell cycle caspase-3 expression

摘要:
Aim: To examine the effect of venom from the spider Macrothele raven on cell proliferation and cytotoxicity in human cervical carcinoma, HeLa cells. Methods: Morphological and biochemical signs of apoptosis appeared using acridine orange-ethidium. bromide (AO/EB) staining. Marked morphological changes in HeLa cells after treatment with spider venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell proliferation and cytotoxicity were determined by [methyl-H-3] thymidine assay ([H-3]TdR) and lactate dehydrogenase (LDH) release, respectively. DNA fragmentation and cell cycle distribution were monitored using flow cytometry. In addition, Western blot analysis was used to evaluate the level of caspase-3 expression. In vivo examination of the inhibition of the size of tumors in nude mice treated with spider venom was measured. Results: Marked morphological changes were observed using AO/EB staining, SEM and TEM assay. Spider venom at concentrations of 10-40 mg/L caused dose- and time-dependent inhibition of HeLa cell proliferation. The ratio of apoptosis and necrosis increased. The activity of caspase-3 was upregulated after spider venom treatment. In vivo study of tumor size revealed that tumors significantly decreased in size from controls to tumors treated for 3 weeks with spider venom (P < 0.05). Conclusion: The inhibition of HeLa cells by the venom of the spider Macrothele raveni was carried out in three ways: induction of apoptosis, necrosis of toxicity damage and direct lysis. Spider venom is a novel anti-tumor material both in vitro and in vivo.

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出版当年[2005]版:
大类 | 4 区 医学
最新[2025]版:
大类 | 2 区 医学
小类 | 1 区 药学 2 区 化学:综合
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出版当年[2005]版:
Q2 CHEMISTRY, MULTIDISCIPLINARY Q4 PHARMACOLOGY & PHARMACY
最新[2024]版:
Q1 CHEMISTRY, MULTIDISCIPLINARY Q1 PHARMACOLOGY & PHARMACY

影响因子: 最新[2024版] 最新五年平均 出版当年[2005版] 出版当年五年平均 出版前一年[2004版] 出版后一年[2006版]

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第一作者机构: [1]College of Life Science, Hebei University, Baoding 071002, China [2]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China [3]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
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通讯机构: [1]College of Life Science, Hebei University, Baoding 071002, China [7]College of Life Science, Agricultural University of Hebei, Baoding 071001, China
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