机构:[1]College of Life Science, Hebei University, Baoding 071002, China[2]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China[3]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.[4]Research Centre, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China河北医科大学第四医院[5]Clinical Laboratory, Bethune International Peace Hospital, Shijiazhuang 050082, China[6]Hebei Provincial Tumor Institute, Shijiazhuang 050011, China[7]College of Life Science, Agricultural University of Hebei, Baoding 071001, China
Aim: To examine the effect of venom from the spider Macrothele raven on cell proliferation and cytotoxicity in human cervical carcinoma, HeLa cells. Methods: Morphological and biochemical signs of apoptosis appeared using acridine orange-ethidium. bromide (AO/EB) staining. Marked morphological changes in HeLa cells after treatment with spider venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell proliferation and cytotoxicity were determined by [methyl-H-3] thymidine assay ([H-3]TdR) and lactate dehydrogenase (LDH) release, respectively. DNA fragmentation and cell cycle distribution were monitored using flow cytometry. In addition, Western blot analysis was used to evaluate the level of caspase-3 expression. In vivo examination of the inhibition of the size of tumors in nude mice treated with spider venom was measured. Results: Marked morphological changes were observed using AO/EB staining, SEM and TEM assay. Spider venom at concentrations of 10-40 mg/L caused dose- and time-dependent inhibition of HeLa cell proliferation. The ratio of apoptosis and necrosis increased. The activity of caspase-3 was upregulated after spider venom treatment. In vivo study of tumor size revealed that tumors significantly decreased in size from controls to tumors treated for 3 weeks with spider venom (P < 0.05). Conclusion: The inhibition of HeLa cells by the venom of the spider Macrothele raveni was carried out in three ways: induction of apoptosis, necrosis of toxicity damage and direct lysis. Spider venom is a novel anti-tumor material both in vitro and in vivo.
基金:
National Natural
Science Foundation of China (No 30371753)
第一作者机构:[1]College of Life Science, Hebei University, Baoding 071002, China[2]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China[3]College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
通讯作者:
通讯机构:[1]College of Life Science, Hebei University, Baoding 071002, China[7]College of Life Science, Agricultural University of Hebei, Baoding 071001, China
推荐引用方式(GB/T 7714):
Gao L,Shan BE,Chen J,et al.Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells[J].ACTA PHARMACOLOGICA SINICA.2005,26(3):369-376.doi:10.1111/j.1745-7254.2005.00052.x.
APA:
Gao, L,Shan, BE,Chen, J,Liu, JH,Song, DX&Zhu, BC.(2005).Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells.ACTA PHARMACOLOGICA SINICA,26,(3)
MLA:
Gao, L,et al."Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells".ACTA PHARMACOLOGICA SINICA 26..3(2005):369-376