高级检索
当前位置: 首页 > 详情页

Protective effects of safranal on hypoxia/reoxygenation-induced injury in H9c2 cardiac myoblasts via the PI3K/AKT/GSK3β signaling pathway.

文献详情

资源类型:
WOS体系:
Pubmed体系:

收录情况: ◇ SCIE ◇ 预警期刊

机构: [1]Department of Traditional Chinese Medicine and Medical History Literature, School of Basic Medicine [2]Department of Traditional Chinese Medicine, School of Pharmacy, Hebei University of Chinese Medicine [3]Department of Pharmacognosy, School of Pharmacy, Hebei Medical University [4]Department of Diagnostics, Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200 [5]Department of Scientific Research Management, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011 [6]Department of Hospital Management and Medical History Literature, Hebei Province Hospital of Chinese Medicine, The First Affiliated Hospital, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China
出处:
ISSN:

关键词: safranal H9c2 cardiac myoblasts anti‑oxidant stress hypoxia/reoxygenation anti‑apoptosis PI3K/AKT/GSK3β signaling pathway

摘要:
Safranal (SFR), an active ingredient extracted from saffron, exhibits a protective effect on the cardiovascular system. However, the mechanism of SFR against hypoxia/reoxygenation (H/R)-induced cardiomyocyte injury has previously not been investigated in vitro. The aim of the present study was therefore to observe the protective effects of SFR on H/R-induced cardiomyocyte injury and to explore its mechanisms. A H/R injury model of H9c2 cardiac myoblasts was established by administering 800 µmol/l CoCl2 to H9c2 cells for 24 h and reoxygenating the cells for 4 h to induce hypoxia. H9c2 cardiac myoblasts were pretreated with SFR for 12 h to evaluate the associated protective effects. A Cell Counting Kit-8 assay was used for cell viability detection, and the expression levels of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), glutathione peroxidase (GSH-px), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and caspase-3, and the intracellular Ca2+ concentration were measured using the corresponding commercial kits. Levels of reactive oxygen species (ROS) in the cells were detected using 2,7-dichlorodihydrofluorescein diacetate. Flow cytometry was used to determine the degree of apoptosis and the level of mitochondrial membrane potential (MMP). Moreover, the expression levels of phosphorylated (p-)PI3K, AKT, p-AKT, glycogen synthase kinase 3β (GSK3β), p-GSK3β, Bcl-2, Bax, caspase-3 and cleaved caspase-3 were measured using western blot analysis. Results of the present study demonstrated that the H9c2 cardiac myoblasts treated with SFR exhibited significantly improved levels of viability and significantly reduced levels of ROS, compared with the H/R group. Furthermore, compared with the H/R group, SFR treatment significantly increased the MMP levels and antioxidant enzyme levels, including CAT, SOD and GSH-px; whereas the levels of CK-MB, LDH, MDA and intracellular Ca2+ concentration were significantly decreased. Moreover, the results of the present study demonstrated that SFR significantly reduced caspase-3, cleaved caspase-3 and Bax protein expression levels, but upregulated the Bcl-2 protein expression levels. SFR also increased the protein expressions of PI3K/AKT/GSK3β. In summary, the results suggested that SFR may exert a protective effect against H/R-induced cardiomyocyte injury, which occurs in connection with the inhibition of oxidative stress and apoptosis via regulation of the PI3K/AKT/GSK3β signaling pathway.Copyright: © Wang et al.

基金:
语种:
被引次数:
WOS:
PubmedID:
中科院分区:
出版当年[2021]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验
JCR分区:
出版当年[2021]版:
Q4 MEDICINE, RESEARCH & EXPERIMENTAL
最新[2024]版:
Q3 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子: 最新[2024版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

第一作者:
第一作者机构: [1]Department of Traditional Chinese Medicine and Medical History Literature, School of Basic Medicine
共同第一作者:
通讯作者:
通讯机构: [5]Department of Scientific Research Management, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011 [6]Department of Hospital Management and Medical History Literature, Hebei Province Hospital of Chinese Medicine, The First Affiliated Hospital, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China [*1]Department of Scientific Research Management, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang, Hebei 050011, P.R. China [*2]Department of Hospital Management and Medical History Literature, Hebei Province Hospital of Chinese Medicine, The First Affiliated Hospital, Hebei University of Chinese Medicine, 389 Zhongshan East Road, Changan, Shijiazhuang, Hebei 050200, P.R. China
推荐引用方式(GB/T 7714):
APA:
MLA:

资源点击量:42329 今日访问量:0 总访问量:1365 更新日期:2025-08-01 建议使用谷歌、火狐浏览器 常见问题

技术支持:重庆聚合科技有限公司 地址:河北省石家庄市健康路12号